5x5x5 LED Cube w/ Animation Software Need Help

That makes sense. I just modified the GUI to send data out over the serial line, this is what it is sending, all on one line:

A2147749888,B1073741824,C537133056,D268435456,E134217728,

Hit me up with a personal message and your email and I can send you the source files and the executable if that would help. From looking at the serial port monitor I am running on the open port the comms look the same if you send them through the serial monitor in arduino IDE as when you send them through the GUI.