Tutorial - How to change firmware on 8u2

Clarification for reply #10. According to the datasheet, the chip looks at the HWB pin state as reset is released (on the rising edge of /Reset). So you should take the wire off the reset pin before taking the wire off the HWB pin. If you take both wires off at once, it's a matter of luck whether the HWB pin is still grounded when you release the reset pin.

So to save frustration, I recommend following the datasheet, and remove the Reset wire before you remove the HWB wire.