interrupts with arduino?

this is what i thought.
i think i will need one timer for my sequencer project (as a stable clock source). is it possible to use one of the timers, when i don't need millis() or pwm output?

OR (even more tricky ;)): can i use the timer interrupts regardless to the fact, that the timers are used for other function too? (in this case i would need to know at which rate the timers are operating, so that i can adjust my needed tempo of the sequencer)