I have a breadboarded Arduino - On my home computer with a RadioShack USB to serial I can load sketches with Arduino-0.22, but Arduino-1.0 i get teh following -
avrdude: Version 5.11, compiled on Sep 2 2011 at 19:38:36
Copyright (c) 2000-2005 Brian Dean, http://www.bdmicro.com/
Copyright (c) 2007-2009 Joerg Wunsch
System wide configuration file is "E:\dld\Arduino\arduino-1.0\hardware/tools/avr/etc/avrdude.conf"
Using Port : \\.\COM6
Using Programmer : arduino
Overriding Baud Rate : 115200
avrdude: Send: 0  
avrdude: Recv: . [ff]
avrdude: stk500_getsync(): not in sync: resp=0xff
avrdude done. Thank you.
At work I have a computer with a hardware serial port and it loads from either 0.22 or 1.0.