FWIW, I do two things before sending a design off to be manufactured. First, take a print of your schematic and go over every connection on the PCB. I use a highlighter to mark off each link. With netlists, this isn't such a big deal, but I find it gives me a chance to eyeball every connection one at a time for things like trace width, proximity, length, better routes, etc.
Second, I print out the board at 1:1, put it over some cardboard, and poke holes into every pin. Then, I put the actual components into the "board" and make sure everything fits without bumping into stuff. It also helps to ensure the holes are the right size for the component leads and so on.