I have disconnected the LED everytime I've tried to program it. The uno is powered by usb. I tried using a 9v battery connected to a 5v 1 amp regulator, with two 10uf caps, one right at the regulator, one right next to the leads for the 85. I'm using premade jumper wires, longest one is about 6 inches. I did not change any wires at all between the first and second sketch except for the one for the positive leg of the led. I removed the uno and then uploaded a simple sketch to the uno, and it worked. Reloaded the isp sketch, same error. I am going to reset this laptop just for kicks....