Upload Timeout Error on Mega 2560 - Not Sure What Changed

hello guys...

i started my own arduino for my project..

after the assembly of entire board, i successfully uploaded the firmware to both atmega16u2 using atmel's FLIP software and atmega2560 as guide from gammon's forum Gammon Forum : Electronics : Microprocessors : Atmega bootloader programmer...

when i tried to upload blink code to my board it shows timout error...

Sketch uses 1,554 bytes (0%) of program storage space. Maximum is 253,952 bytes.
Global variables use 9 bytes (0%) of dynamic memory, leaving 8,183 bytes for local variables. Maximum is 8,192 bytes.
C:\Program Files\Arduino\hardware\tools\avr/bin/avrdude -CC:\Program Files\Arduino\hardware\tools\avr/etc/avrdude.conf -v -patmega2560 -cwiring -PCOM16 -b115200 -D -Uflash:w:C:\DOCUME~1\Krishna\LOCALS~1\Temp\build08e4957f02c018eb579e11ddc64f9701.tmp/Blink.ino.hex:i

avrdude: Version 6.0.1, compiled on Apr 15 2015 at 19:59:58
Copyright (c) 2000-2005 Brian Dean, http://www.bdmicro.com/
Copyright (c) 2007-2009 Joerg Wunsch

System wide configuration file is "C:\Program Files\Arduino\hardware\tools\avr/etc/avrdude.conf"

Using Port : COM16
Using Programmer : wiring
Overriding Baud Rate : 115200
avrdude: stk500v2_ReceiveMessage(): timeout
avrdude: stk500v2_ReceiveMessage(): timeout
avrdude: stk500v2_ReceiveMessage(): timeout
avrdude: stk500v2_ReceiveMessage(): timeout
avrdude: stk500v2_ReceiveMessage(): timeout
avrdude: stk500v2_ReceiveMessage(): timeout
avrdude: stk500v2_getsync(): timeout communicating with programmer

avrdude done. Thank you.

can anyone help me in solving this problem weather it is hardware or software side
........

firmware for atmega16u2 is "arduino_dfuserial.hex"