Sorry to bring this topic from the dead but im searching the net for the making of a bee venom machine and this came into so i m posting here for some extra info if you can provide me. From my search on the net i found this usefull info from a patent device:
"In accordance with the present invention, the controller is supplied with an input voltage of DC 12V, with an output voltage elevated to AC 12V to 40V at an output frequency from 30 to 400 Hz."
As i read here and all over the net a 555 circuit is the answer. Adding a step up converter after the 555 circuit wont it scramble the final output frequency ? Some simple ebay converters say that the output voltage will have 400khz frequency.