Do you have a bootloader in your AVR chip? You need one.
Check the board is working as a chip with just the boot loader in will flash the LED on Pin13, if you don't have a LED here just look at the pin on an oscilloscope.
version arduino software is used
I would use the latest.
and pc configaration also
If you are using the built in COM ports then nothing to configure.
but it's not working.
So what messages do you get when you try?