Not sure if this will help anyone else, but with the 3d printer kit I purchased it had me install the Repetier Software which has a server and host component. The server was constantly connecting to the arduino and preventing me from being able to upload.
The usual culprit is a mobile phone / PDA application running in the background that constantly probes for a connected device.
Hi,i just purchased an ezlcd 303 with Uno on it. There is no respond and handshake during test on Termie (code like CLS, PING) despite COM port, baud rate has been checked.For IDE test i get the following error:avrdude: ser_open(): can't open device "\\.\COM3": Access is denied. Thanks.
In my case, none of this worked. Turns out the problem was that Windows Update for Win10 "updated" my USB host controller and the new driver malfunctioned, but only in a few specific cases, the use of an Arduino Uno being one of them. The fix was to uninstall the driver and reinstall the manufacturer-supplied driver. Just one more thing to look at in the event you have this issue.RD7