Suddenly not possible to upload to real Nano

Thank you! Maybe it is a problem in W10?

I tried now on desktop program and get this:

Arduino:1.8.8 (Windows 10), Kort:"Arduino Nano, ATmega328P"

Sketch uses 930 bytes (3%) of program storage space. Maximum is 30720 bytes.
Global variables use 9 bytes (0%) of dynamic memory, leaving 2039 bytes for local variables. Maximum is 2048 bytes.
Sketch uses 930 bytes (3%) of program storage space. Maximum is 30720 bytes.
Global variables use 9 bytes (0%) of dynamic memory, leaving 2039 bytes for local variables. Maximum is 2048 bytes.
avrdude: ser_open(): can't open device "\\.\COM12": Åtkomst nekad.


Problem vid uppladdning till brädan. Se http://www.arduino.cc/en/Guide/Troubleshooting#upload för förslag.

This report would have more information with
"Show verbose output during compilation"
option enabled in File -> Preferences.