Maybe, how can I find out?
You probably have to explicitly erase or replace the bootloader code to get rid of it.
What sort of startup time do you need to achieve?For example, there's a ~0.5s pause while the bootloader checks for "double tap reset", and (I guess) a longer pause waiting for traffic. One elegant solution would be to change the startup to ONLY enter the bootloader on the double-tap...
you have to create a custom variant with a different linker script putting the binary together to address 0.