yes, and if you have your uno setup as an ISP you dont even really need to swap chips, the same setup that allows you to use an arduino to upload the bootloader can be used as a direct programmer too (which then you dont even need the boot loader in the first place)
as long as you have one chip with a bootloader, and the means to connect it to your pc (ie an arduino board), your good to go for bootloading or not on your target chip