Arduino Robot connection problem


I have one problem with my Arduino Robot Control board.

I can't download program to the board from arduino studio. When i was compiling my last program i've notice that i didnt plug usb cable so i plug it in before program started downloading.

After this, program on control board is working but i can't download any other program to board. when i try it i get this message:

avrdude: error: programm enable: target doesn't answer. 1  
avrdude: initialization failed, rc=-1 
     Double check connections and try again, or use -F to override 
     this check.

I thought it could be problem with bootloader but i cant burn new bootloader, ive tried even with usbasp through ISP but it didnt work.

Can someone help me?