Yup. Unless you like hugh paths, copy C:\Users\spcomputing\Desktop\arduino-1.0.1\hardware\arduino\firmwares\UNO-dfu_and_usbserial_combined.hex to C:\Users\spcomputing\Desktop\arduino-1.0.1\hardware\tools\avr\bin.
Hook the USBTiny to the 3x2 header behind the USB port with the red side of the ribbon towards pin 1/2 (furthest from the USB port).
Open a command prompt in C:\Users\spcomputing\Desktop\arduino-1.0.1\hardware\tools\avr\bin.
Type:
avrdude -p at90usb82 -F -P usb -c usbtiny -U flash:w:UNO-dfu_and_usbserial_combined.hex -U lfuse:w:0xFF:m -U hfuse:w:0xD9:m -U efuse:w:0xF4:m -U lock:w:0x0F:m