ATMega2560 + 16U2 MIDI Trouble

Hello I am developing a MIDI controller with a PCB that I have designed, it is not my first full design based on this two microcontrollers for a MIDI device but I am having some troubles with this one.
The problem is that when I program it(only one potentiometer of the 10 that it has to try that it works) it doesn't send the correct MIDI messages to the PC.

I have this on mi 2560

#include <MIDI.h>

MIDI_CREATE_DEFAULT_INSTANCE();
using namespace midi;

void setup()
{
  Serial.begin(31250);
  MIDI.begin(2);
}

void loop()
{
  MIDI.sendRealTime(ActiveSensing) ; 
  MIDI.read();
  int pot1 = analogRead(A0);
  int val1 = map(pot1, 0, 1023, 0, 127);
  MIDI.sendControlChange(1, val1, 2);    //GAIN A        // Control Number, Control Value, Channel
  int pot2 = analogRead(A1);
  int val2 = map(pot2, 0, 1023, 0, 127);
  MIDI.sendControlChange(2, val2, 2);    //HIGH A
  int pot3 = analogRead(A2);
  int val3 = map(pot3, 0, 1023, 0, 127);
  MIDI.sendControlChange(3, val3, 2);    //MID A
  int pot4 = analogRead(A3);
  int val4 = map(pot4, 0, 1023, 0, 127);
  MIDI.sendControlChange(4, val4, 2);    //BASS A
  int pot5 = analogRead(A4);
  int val5 = map(pot5, 0, 1023, 0, 127);
  MIDI.sendControlChange(5, val5, 2);    //FILTER A
  int pot6 = analogRead(A5);
  int val6 = map(pot6, 0, 1023, 0, 127);
  MIDI.sendControlChange(6, val6, 2);    //VOLUME A
}

then it is conected throught serial with the 16U2 that has a MOCO_Lufa bootloader to act like a HIC device.

As I have said it isn't my first MIDI controller that I have made on a PCB, but it is the first that is causing troubles with this.

I have tried only with MIDI.begin(MIDI_CHANNEL_OMNI); then changed it to work only at channel 2, I after added the Serial.begin(31250); because I rode that it could be a sync trouble, I added the MIDI.sendRealTime(ActiveSensing) ; because in a first instance, the MIDI OX Monitor only rode RT messages and anythingat other channels.

I have rechecked all the crystal oscillators and capacitors, but this can't be a problem because I have no problem to upload the sketches to the 2560 or to reburn the bootloader of the 16U2.

So I don't know what can it be or how to solve.

Have anyone experienced something similar?

Thank you so much
Best regards