Automatic Clamping advice

Zach_8r3:
Forgot to mention I'm trying to avoid peristaltic pumps do to slowness. And I don't want to have to buy a whole bunch of them to compensate.

3/16" dia tubing will be slow unless you spend some money on powerful pumps.
as for backlow, just keep the discharge above the top of the target vessel, zero possibility of backflow