avrdude - programming attiny85 using mega2560 as ISP

I am trying to upload the micronucleus bootloader to an attiny85 using avrdude and mega2560 as the isp. I have not yet tried to upload since I have been unable to verify a connection between my computer and attiny85 using avrdude.

In cmd I have tried: .../avrdude -C avrdude.conf -c arduino -p t85 -P com7
and get the response:
avrdude: stk500_recv(): rogrammer is not responding
avrdude: stk 500_getsync() attempt 1 of 10: not in sync: resp=0x00
then repeats, however sometimes it is: "resp= 0xe0" at the end

I have double checked the connections and I am still able to upload code to the attiny using arduino IDE.

Any help would be appreciated