Change Assembly to Arduino IDE

Whether they are in the (quite limited) address space accessible by the sbi/cbi instructions...

use

constexpr uint8_t led {13};

From reading the rest of your posts, it seems that maybe you weren't interested in assembly language after all, but in case you are interested, or in case others who are interested find this thread, I will add that, in addition to the option of using inline assembly in your .ino files via the asm keyword as jim-p already mentioned, you can also add pure assembly files to your sketch.

There is a simple "hello world" demonstration of that here:

the value will be evaluated during compile time, whereof in case of a const uint8_t you rely on the optimization of the compiler. So there might be edge cases where the compiler optimization of a const variable could fail and end up in RAM, but constexpr variables will be inserted as literals (otherwise it will not compile).

I'm not good in such things, better read:
https://en.cppreference.com/w/cpp/language/constexpr