Does being at high altitude may affect the throttle input in rise of quad?

I get stable system. like it doesn't when I am trying to lift of even after 40-50% it remains quite stable but doesn't lift off.

I am still suspicious about which props you have mounted on which motor. They must, of course, match the motor rotation.

For a normal quad, what is usual throttle input to get lift off. I understand it is depends up on weight but on average after what throttle input will quad start to hover?

There are too many variables to be able to provide a sensible answer.

One of my 250 quads lifts off at about 1/4 throttle whilst the other lifts off at 1/2 throttle, Different motors and different props on each. The throttle input needed to lift off is irrelevant if you think about it.