Has it ever worked? -- Or, are you trying to get it to work for the first time.
What version of the IDE (1.0.2?)
Is it a new Uno "R3" (says on the board)?
I haven't seen this problem in a while but back when I did it was either a driver install problem (shouldn't be a problem with the newest boards), or just a Windows/Usb issue, which needed something like:
- Unplug Arduino from USB.
- Exit Arduino IDE.
- Wait until the device doesn't show up any more in Windows (5-10 seconds, depending how fast the computer is)
- Plug the board back in, wait for the device to show up in Windows.
- Launch the Arduino IDE.
- Try uploading.
And/or try PC reboot between steps 3 and 4.
Good luck!
John