FardinB:
So my question is what is the fastest method to send 5 data to the computer? to make matters worse, to capture two of these data I have to do serial comm with the two temp sensor breakout boards.
You haven't mentioned the break-out boards until now. How are they connected? What serial settings and protocol do they use? Can they actually provide samples at a sustained 1KHz?
To send the results to the PC you will need to whack the serial port speed up, use a concise message format, and hope for the best. I don't know whether you'll be able to achieve 1 KHz sample rates, but perhaps you will manage it.