What is the project that you were working on when it started to fail?
Just some points:
- Which operating system are you using?
- Did you select the correct port?
- What is the serial-to-usb converter on your board?
An official Uno will have a 16U2, clones might have a CH340 or other chip.
If your board does not use the CH340, have you done the loopback test? What are / were the results? - Is the board properly detected by the operating system?
- Anything connected to pins 0 and 1? Remove it and try again. If that fails, remove anything connected to the board (except PC) and try again.