Hello, I am trying to figure out if it is possible to measure the barrier function of a cell monolayer in a transwell using an arduino. Normally you would use a very expensive TEER measuring device which is essentially a pair of chopsticks, which pass a current through the media, and the resistance gives you a value for the strength of your cellular barrier. When you measure this over a few days, you should see an increase in resistance, and that's your barrier. You can then throw in compounds to disrupt this barrier etc etc.
Whilst in previous labs we did have this equipment, my current one doesn't and we can't afford to buy one, so we are using a less sensitive method involving labelled molecules.
I'm not an expert in electronics/arduino, but Im wondering if anyone has attempted to mimic TEER equipment with an arduino? I have read a number of papers where people are using fabricated chips connected to arduino, but we need to use transwell/chopstick method.
If someone has already tried this, i'd love to know if it's worth it!