Programming

After testing my sketch on Arduino , can I burn HEX file generated in Arduino environment in to uC not having bootloader .with AVR ISP or equivalent

Yes you can... assuming it is the same processor. You'll have to search for the HEX file, it is in a temp folder somewhere.

Thanks

You can take a look at this post to see how to upload the hex file manually:

http://www.arduino.cc/cgi-bin/yabb2/YaBB.pl?num=1265576847