Rev 4 of a complex PCB and suddenly I have a weird problem I can't understand

Thanks very much for the reply! How would I go about tracing it?

If I put my multimeter across 12v and GND I get no reading on ohms, but also no beep on continuity (though it does show 1.007 - not sure what units this is though).

From 5v output on the DC-DC converter to GND I get 16k ohms and no beep on continuity but 0.434 on the screen (again don't know units for that).

How might I go about tracing the short?