How can I separate the variables with the arduino using serialRead() ?
You can't separate them using Serial.read(). To do that, you need to collect the data into an array, and then use strtok or sscanf to extract the parts of interest.
char array[] = "one, two";
char *token = strtok(array, ",");
// token will be "one"
token = strtok(NULL,"\0"); // Use NULL to keep parsing the same array, and \0 to get the rest
// token will be "two"