Third Serial on Nano 33 BLE (can be software)

Official Hardware Nano Family Nano 33 BLE
5

Did you try the procedure given on the GitHub link.

Try add as a header above setup

UART mySerial(digitalPinToPinName(4), digitalPinToPinName(3), NC, NC);  //4 is tx, 3 is rx
//(change 4 and 3 as you prefer, the processor can mux any functionality on any pin)

Then in setup
mySerial.begin( ); //Add baud rate

You should be able to have Serial, Serial1 and mySerial on the Nano33BLE.

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