Waveshare ILI9486 TFT 3.5 INCH LCD DISPLAY not working on a MKR WIFI 1010 put on a MKR2UNO adapter

No, I have not studied your MKR2UNO pinout.

But I would just guess that MOSI, SCK will be found on the 3x2 SPI header.
Make sure that the SPI_Config jumpers are open.

And you can use any GPIO pins for LCD_CS, LCD_DC, LCD_RST, LCD_BL. But obviously easier if you select the same ones as on the display.

David.