What is the best mechanism for controlling a mouse-size door on a tube?

I’m not actually sure I need an arduino, I would actually prefer to go with a mechanical solution. But what I can’t figure out is how to mechanically open the door to release the mycelium without introducing contamination.

ie, if I have a stick built into the top lid, and push down into the tube to push a spring-loaded bottom lid open, that stick may introduce contaminants as it slides down.

If the tube is filled with UV-C LED’s it may potentially be a non-issue though. The most important aspect of mycology is preventing contamination and UV-C isn’t instant; ie it needs a few seconds to fully kill mold and bacteria.