Geez, I haven't thought about that for a LONG time. Just counting the languages I wrote at least one substantial program in, hmmmm:
Fortran II, IV, WATFOR, Ratfor
Algol (several variants)
PL/1, PL/C & PL/M
RPG
APL
SNOBOL
COBOL
BCPL
SAIL
PASCAL
BASIC (lots of variants, including VB)
LOGO
SPL (for HP3000)
C/C++/Objective C
Smalltalk
Forth
LISP (several variants)
Bliss
Ada
Modula
Mesa
Java/Javascript
Not sure i could make a list of all the assemblers I've had to deal with. Includes at least:
IBM 11/30 and 360/whatever, Autocoder for 1401
Univac 1108
PDP-8/10/11, DG NOVA, HP2100
Intel 4004, 8008, 8068, 80386, Zilog Z8, Z80, MOS 6502 and variants
Mot 6800, 68000, 68010, 68020 and variants, Signetics 2650
Various Microcoded processors including a couple of PDP-11 models, Alto, D1/D2, Perq and a couple of other 2900 and Intel 3000 series machines
Not sure if I count shell/macro languages (csh, teco, emacs and the like), since it's hard, but not impossible to write a substantial program in them, and I usually only did quick hacks.
Hey that was a trip down memory lane. And I'm not a programmer by trade.