SANDIE:
Avrdude: warning: cannot set sck period. please check for usbasp firmware update.is this warning is problem ???
No, please ignore it. The stock firmware on your USBasp will work fine. That won't cause any problems and trying to update the firmware may cause you problems. It's best not to worry about it unless you really feel like trying out a non-standard firmware but even then you should wait until you have all the other worked out and a backup USBasp or two on hand.
SANDIE:
is any way to do set 16 mhz without using bootloader?????
Yes, but it's more work and more complicated. You would simply use AVRDUDE from the command line to set the fuses however you want. I'd rather just do Tools > Burn Bootloader to set the fuses even if I'm just going to immediately overwrite the bootloader doing Sketch > Upload Using Programmer.