Uploading sketches suddenly was not possible anymore. Cable, computer, board, software, IDE ,port , computer: everything was checked. Last thing I thought of: the board manager
The update for Arduino AVR board in board manager 1.6.21 does not work. It gives a time out on my MacBook. I switched back to 1.6.20 and it works again. Please be aware of this!
Thanks! I did try this solution yesterday, but I got an error message (upload error). Because of your advice I tried a few times more today and now it works, thanks!