buffer_overfly:
What voltage level or levels would trigger the ISR? Will it be running as long as V(A0) crosses that level, or will it stop after being run once?
A pin change interrupt is triggered when the voltage changes between 0 and 1, in either direction. The voltage level that a 0->1 transition occurs isn't well defined. Atmel guarantees level 1 at a minimum of 0.6Vcc, or 3V for a processor running at 5V. But it will likely be something less than that. If you want a more well defined threshold you should use the comparator to generate an interrupt. That's what it's there for.