I recently was directed to this site http://oshpark.com/, it may have been someone on this site who told me about it. The cost for a two layer board is $5 per sq inch for three boards, shipping is included. Boards must be ordered in multiples of 3, your boards are panelized with others they seem to have the volume to send out a panel each day.
I sent them a simple design for a bare bones ATTINY2313 header board, and 10 days later I had the boards in hand and they looked and functioned great. Today I sent them a design for an instrumentation amp board for a project that included a load cell, the charge on these three boards was $4.44. I would have ordered more than 3 of the instrumentation boards except it was a new design and it may need some mods. No loss as anytime I want more of any boards I have purchased from them the files are on their site and I can log in and place an order anytime I want.
They also do 4-layer boards, the cost is more and I do not think they send a panel out each day, if interested check their site out.