Heater for PCR Process [Extreme Temperature Control]

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1

Hi there,

I'm working in a project to do a similar PCR technique. That means Polymerase chain reaction and is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.

I imagine that you thought, what are you talking about? But the process consist in heating a surface to 65°C (+0°C and - 0.5°C) for 45 min. The problem is the heater that I've used, originally a Peltier Cell but it does not exceeds 50°C.

To control the temperature process I've used a PID.

Someone can tell me an idea? The homogeneous temperature is the most important thing here and the surface is like image below.

Thanks in advance.
Omar.

2

The image is not displaying.

You know you can heat the 'cold' side of the Peltier and get a hotter 'hot' side? You can even stack Peltiers, but the efficiency is very poor.

3

HI,
If you use REPLY and not QUICK REPLY you will find on the lower left of screen an ATTACHMENT function, this will allow you to attach images and sketches if they are to big of the editor.

Tom.... :slight_smile: